Stable Cell Line Selection Marker

The marker gene could be on either the same plasmid vector or a second co transfected vector.

Stable cell line selection marker.

Evaluating the influence of selection markers on obtaining selected pools and stable cell lines in human cells selection markers are common genetic elements used in recombinant cell line development. As a negative control cells should be transfected using dna that does not contain the selectable marker. Selection of stable transfectants selection of stably transfected cells begins with successful transient transfection with a plasmid containing a selectable marker such as an antibiotic resistance gene. Selection marker in order to select stably transfected cells a selection marker must be co expressed with the target protein.

Stable cell line generation is made possible by the use of positive selection markers such as hygromycin g418 geneticin zeocin and blasticidin antibiotic resistance. Selection markers can be delivered using the same plasmid that contains the gene of interest in cis or on a separate plasmid in trans that needs to be co transfected with the plasmid containing the gene of interest. Selection markers are common genetic elements used in recombinant cell line development.