Str Profiling For Human Cell Line Authentication

The cell line authentication testing division of labcorp formerly known as genetica cell line testing a labcorp brand provides reliable fast and affordable authentication of human cancer cell lines stem cell lines xenografts and more utilizing str dna technology.

Str profiling for human cell line authentication.

Nih ncbi atcc iclac recommends validation of cell lines for grant applications and journals. This document elaborates a standardized procedure for unambiguous authentication and identification of human cell lines using str profiling. Grcf dna services offers cell line authentication analysis following ansi atcc asn 0002 2011 authentication of human cell lines. The profiling kits are only suitable for cell lines of human origin.

Real time qpcr mycoplasma contamination detection. This effort has facilitated the broader adoption of cell line authentication testing into cell culture laboratory operations. Mouse cell line authentication via str profiling. Short tandem repeat str dna profiling is one solution that has been proposed for the detection of cross contamination and therefore authentication of human cell lines.

A beta globin mouse marker option is offered to test for the presence of mouse dna. A growing list of 70 journals and organizations are now highly recommending or requiring cell authentication to publish a researcher s findings. It helps to detect misidentified genetically drifted or cross contaminated cells that invalidate research results. After determining the str profiles of your human cell lines you may compare them to the human cell lines in the atcc str database.

As such establishing a human cell line s identity through short tandem repeat str profiling has become essential in conducting valid reproducible impactful research. Results available online typically in 2 business days includes mouse marker. The overall objective of this standard is the specification of the methodology for dna extraction and for str profiling data analyses quality control of the data interpretation of results and. The standard also provides guidance on the interpretation of str profiling results.

The cell line integrated molecular authentication clima database is the result of an integration effort aimed at including all certified str profiles of human cell lines in a unique database. We offer complete cell line authentication services using short tandem repeat str profiling. Str profiling aids in the detection of misidentified cross contaminated or genetically drifted cells which invalidate research results. Standardization of str profiling guidelines using multiple kits.

Its main feature is cell line identification according to the.